Extended Data Fig. 4: TH9619 and TH9975 impair GMP synthesis in SW620 MTHFD2-/- cells and reduce IMP levels in SW620 WT and MTHFD2-/- cells.
From: Formate overflow drives toxic folate trapping in MTHFD1 inhibited cancer cells
(a–d) [U-13C]serine- derived MID of GMP (a) and IMP (c) and levels of GMP (b) and IMP (d) normalized to packed cell volume (PCV) in SW620 WT and MTHFD2-/- cells treated for 24 h with 1 μM TH9619 or TH9975, 50 μM hypoxanthine and 50 μM thymidine (cultured in RPMI-dFBS), means ± SD (n = 3); one-way ANOVA with Šídák’s multiple comparisons test. For panels a and c, statistical analysis was performed for M + 0. P-values indicate comparisons to respective DMSO controls.
