Extended Data Fig. 3: Genotyping for XOR variants in mGECs and FACS gating strategy for ROS.
From: Genetic susceptibility to diabetic kidney disease is linked to promoter variants of XOR
a) Representative agarose gel showing bands for B6-XorWT (top) and D2-WT (bottom) variants. Molecular weight markers in lane 1, lane 2: D2-WT, lane 3: B6-XorWT mice and in lane 4: mGECs. Image is representative of 4 independent tests. b) Genotyping strategy for detecting SNP variant through mismatch with AS primers F1 and F2 using a common R primer as described in Methods. c) FACS gating strategy showing a representative plot of negative unlabeled control mGECs % population of CM-H2-DCFDA, and d) % population of cells positive for CM-H2-DCFDA after 24 hr HG treatment.
