Extended Data Fig. 5: SM DBT knockout is specific and does not affect body weight, muscle physiology, or glucose tolerance on chow diet.

a-d, Gene expression data via qPCR of Dbt, Dbt exon 6 (deleted region), Bckdha, and Bckdhb, respectively; n = 3 Dbt^l/l (WT) and n = 5 Dbt^l/l-HSA-CreER (Cre). e-g, Western quantification of DBT in fasted tissues normalized to loading control; n = 3 WT and n = 3 Cre. h, H&E staining of tibialis anterior (TA) muscle. Representative images from n = 3 WT and n = 3 Cre. I, Ratio of 3-HIB/valine, and j, Concentration of 3-HIB from mice fasted for 5-hr (n = 10 WT and n = 8 Cre) or re-fed for 2-hr after an overnight fast (n = 11 WT and n = 9 Cre). k, Body weights; n = 10 WT and n = 8 Cre. l, 2 g/kg glucose IP-GTT in 5-hr fasted mice; n = 10 WT and n = 10 Cre. m, Area-under-the-curve (AUC) for the GTT in l. n, Insulin concentration at t = 0 and t = 20 min (n = 10 WT and n = 7 Cre) during GTT from l. Mice used in these experiments were male, aged 10–19 weeks fed normal chow. Data are presented as means ± SEM. Comparisons of two groups use two-tailed Student’s t-test with significance defined as: *p < 0.05 and ***p < 0.001. Experiments with multiple comparisons at different time-points use two-way ANOVA with repeated measures. Exact p-values: a, p = 0.039254. b, p = 0.043912. f, p = 0.000749. g, p = 0.011125.