Extended Data Fig. 8: Liver DBT knockout does not affect body weight or glucose tolerance on multiple diets or after a BCAA challenge.

a-c, Western quantification of DBT in tissues normalized to loading control; n = 3 Dbt^l/l-AAV8:GFP (GFP) and n = 3 Dbt^l/l-AAV8:Cre (Cre). d, Body weights of n = 10 GFP and n = 10 Cre mice on chow and HFD. e, Plasma labeling of BCAAs after U¹³C-BCAA gavage (fraction); n = 4 GFP and n = 5 Cre. Labeling fraction of TCA cycle intermediates 10 min post U¹³C-BCAA gavage in f, quad g, iBAT, & h, gWAT in 5-hr fasted mice; n = 4 GFP and n = 5 Cre. i, BCKDH complex activity measured in fasted livers from n = 4 GFP and n = 4 Cre mice. j, 1.5 g/kg glucose IP-GTT in 5-hr fasted mice fed HFD for 4 weeks after a BCAA gavage given at t = 0; n = 8 GFP and n = 9 Cre. k, Area-under-the-curve (AUC) for the GTT in j. l, Insulin concentration at t = 0 and t = 20 min (n = 8 GFP and n = 9 Cre) during GTT from j. m, Plasma BCAA concentration in 5-hr fasted mice before (t = 0) and 20 min after (t = 20) a gavage of BCAAs from GTT + BCAA gavage in j; n = 8 GFP and n = 9 Cre. Mice used in these experiments were male, aged 16–24 weeks and fed normal chow for 4–5 weeks, followed by HFD for 4–6 weeks. Data are presented as means ± SEM. Comparisons of two groups use two-tailed Student’s t-test with significance defined as: *p < 0.05. Experiments with multiple comparisons at different time-points use two-way ANOVA with repeated measures. Exact p-values: b, p = 0.025494. m, valine t = 0 p = 0.031669, leucine t = 0 p = 0.033539.