Extended Data Fig. 7: Mettl3 deficiency in hepatocytes causes mitochondrial damage and ER stress.
From: m6A modification-tuned sphingolipid metabolism regulates postnatal liver development in male mice
a, Membrane fluidity of primary hepatocytes from WT and Mettl3ΔHep mice determined by TMA-DPH (n = 7 biological independent samples). b, Electron microscopy of WT and Mettl3ΔHep livers. Arrowheads indicate the perinuclear space. Scale bar, 5 μm. c, Mitochondrial membrane potential assessment of primiary hepatocytes from WT and Mettl3ΔHep mice 24 hours after isolation with the mitochondria-specific probe JC-1. Red and green fluorescence indicate J-aggregates and JC-1 monomers, respectively (n = 3 biological independent samples). Scale bar, 100 μm. d, Mitochondrial membrane potential assessment of primiary hepatocytes from WT and Mettl3ΔHep mice 48 hours after isolation with the mitochondria-specific probe JC-1. Red and green fluorescence indicate J-aggregates and JC-1 monomers, respectively (n = 3 biological independent samples). Scale bar, 100 μm. **p < 0.01 by unpaired two-tailed Student’s t test.
