Extended Data Fig. 4: CRAT deficiency induces type I interferon responses in NRVMs.
From: CRAT links cholesterol metabolism to innate immune responses in the heart
a-e, NRVMs were transduced with lentivirus expressing Crat shRNA, control shRNA or no virus for 3 or 5 days. RNAs were extracted from the cells and RT-PCRs were then performed to determine the expression of Aim2 (a), Ddx58 (b), Ifih1(c), Ifit3 (d) and Irf7 (e). n = 4 (a-e) biologically independent samples per group. Data are shown as the mean ± SEM. Analysis was two-way ANOVA followed by Bonferroni’s multiple comparison test (a-e).
