Extended Data Fig. 9: PPARs inhibitor, GW9662, inhibits the type I interferon responses in CRAT-deficient NRVMs. | Nature Metabolism

Extended Data Fig. 9: PPARs inhibitor, GW9662, inhibits the type I interferon responses in CRAT-deficient NRVMs.

From: CRAT links cholesterol metabolism to innate immune responses in the heart

Extended Data Fig. 9

NRVMs were transduced with lentivirus expressing control shRNA or Crat shRNA. Cells were then treated with GW9662 (10 μM) or T0070907 (10 μM) as indicated for two days. a, b, RT-PCR indicated that GW9662 inhibited the induction of Cyp27a1 (a) and Cyp7b1(b) in CRAT-deficient NRVMs. c, d, GW9662 but not T0070907 abolished the increase in ISG expression induced by CRAT depletion. n = 6 (a-c), 4 (d) biologically independent samples per group. Data are shown as the mean ± SEM. Analysis was two-way ANOVA (a-b) or one-way ANOVA (d) followed by Bonferroni’s multiple comparison test and unpaired two-tailed Student’s t-test (c). NS, not significant.

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