Extended Data Fig. 10: Mitochondrial DNA activates AIM2-inflammasome in vitro.
From: CRAT links cholesterol metabolism to innate immune responses in the heart
Purified GST–AIM2 (2 μg), ASC protein (1 μg), His-Caspase-1 (2 μg) were incubated in the presence or absence of mtDNA (1 μg) or poly (dA:dT) (1 μg) in TE buffer for 30 min at 37 °C. The reaction mixtures were then loaded on SDS–PAGE gel and Western-blots were performed with the indicated antibodies. Here is the representative result from three independent experiments.
