Extended Data Fig. 4: ENO2 regulates malignant behavior and phenotype in CRC cells.
a, b, The effect of ENO2 on the migration and invasion of CRC cells was determined by (a) Transwell migration and (b) invasion assays. Quantification of the numbers of (a) migrated and (b) invaded cells is shown. Scale bar, 100 μm. c, HUVECs were treated with recombinant human ENO2 protein (20 ng/mL), and tube formation assay was performed. Quantification of the number of endothelial tubular structures. Scale bar, 200 μm. rhENO2, recombinant human ENO2 protein. (a-c, representative of n = 3 biological replicates). d, The effect of ENO2 on the expression of proangiogenic factors in HCT116 cells were assessed by qPCR assay. n = 3 biologically independent experiments. e, f, The effect of ENO2 on the expression of EMT- and stemness-related markers in CRC cells was evaluated by (e) Western blotting and (f) qPCR assay. Fold change of mRNA level in each group is shown. (e, representative of n = 3 independent replicates. f, n = 3 biologically independent experiments). Data are presented as the mean ± s.e.m. P values were determined by unpaired two-tailed Student’s t-test (a, b, ENO2-transfected group; c; d; f left) or one-way ANOVA with Tukey’s multiple comparison test (a, b, siENO2-transfected group; f right).
