Extended Data Fig. 5: CAPN15 is the downstream transcriptional factor of TMEM164. (Related to Fig. 3).
a, Protein concentrations of CXCL1, CXCL2, and CXCL10 in the culture medium of EGFP-PBS, EGFP-ITC, and TMEM164-ITC astrocytes were measured by ELISA, n = 3 replicates per group. b, Relative mRNA expression levels of C3 among the groups of astrocytes with TMEM164 overexpression and individual transcriptional factor co-overexpression, n = 3 replicates per group. c, Relative mRNA expression levels of Tmem164, Capn15, and Elovl1 in primary mouse astrocytes treated with PBS or ITC, n = 3 replicates per group. d, Relative mRNA expression levels of Capn15 when knocking down Capn15 using CasRx/gRNA system, n = 5 replicates per group. e, Schematic illustration of the possible mechanism underlying TMEM164 overexpression-mediated suppression of neuronal death. Created with BioRender.com. All data were presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, p values were provided in Source data, one-way ANOVA and Tukey’s multiple comparison test in panels a, b, and d; unpaired two-tailed student’s t-test in panel c.
