Extended Data Fig. 6: Effect of ced-3(-) and fasn-1(D/E) mutation on metabolism with diverse stressors.
From: Proteolytic activation of fatty acid synthase signals pan-stress resolution
a, Pearson’s analysis of metabolites changed in fasn-1(D/E) and ced-3(-) null mutants compared to wild type animals with no stress treatment. b, eigenvalues of PCA analysis for wild type, ced-3(-) and fasn-1(D/E) mutant with no stress treatment and each of the ER, osmotic and ROS stress. c, images and quantification of Grx1-roGFP2 ratio in wild type, ced-3(-) and fasn-1(D/E) under no stress condition. Excitation of 408 nm was used to estimate GSSG and excitation of 488 nm was used to estimate GSH. Ratio-metric images of the two channels was generated using Fiji (Image J v 1.54 f). Scale bar, 200μm. WT n = 17, ced-3(-) and fasn-1(D/E) n = 20. d-e, heatmap of detected metabolites in carnitine synthesis (d) and beta-alanine metabolism (e) pathway. Log2-fold change relative to mean value of wild type no stress. n = 6 biological replicates. f, total caspase-3 protein detected in lysate. Average of vehicle treated set to 1, n = 3. Mean ± SD. g, GSSG/GSH ratio of wild type animals under diverse stress conditions. n = 6 biological replicates. Mean ± SD. Data from untargeted metabolomics. p values were calculated using Mann-Whitney, two-tailed test (c,g) or unpaired, two-tailed t-test (f).
