Extended Data Fig. 7: Extended Data Fig.7 Cellular oxidative state alters caspase-mediated stress responsiveness.
From: Proteolytic activation of fatty acid synthase signals pan-stress resolution
a, representative images of HSP-4p::GFP stress reporter induction under ER stress with N-acetylcysteine (NAC) supplementation in wild type animals. Quantification n = 30 animals. b, representative images of HSP-4p::GFP stress reporter induction under ER stress with NAC supplementation in ced-3(-) animals. Quantification shown in main Fig. 3. c, representative images of HSP-4p::GFP stress reporter induction under ER stress with carnosine supplementation in wild type animals. Quantification n = 30 animals. d, representative images of HSP-4p::GFP stress reporter induction under ER stress with carnosine supplementation in ced-3(-) animals. Quantification shown in main Fig. 3. e-f, representative images of HSP-4p::GFP stress reporter induction under ER stress with MDA (malondialdehyde) supplementation in wild type animals (e) and ced-3(-) animals (f). Quantification shown in main Fig. 3. g-h, representative images (g) and quantification (h) of HSP-4p::GFP stress reporter induction under increasing tunicamycin concentration with MDA (malondialdehyde) supplementation in ced-3(-) animals. n = 40 animals. p values were calculated using Mann-Whitney, two-tailed test (a,c,h). Scale bar, 200μm (a,b,c,d,e,f,g).
