Extended Data Fig. 8: Impact of FASN-CTF on gene expression and metabolic profiles.
From: Proteolytic activation of fatty acid synthase signals pan-stress resolution
a, diagram showing the ___location of FASN-NTF linker region which is a degron. NTFΔ is FASN-NTF with the linker region removed. b, Western blot of FASN-NTF showing the band near 150 kDa corresponds to removal of the linker region (NTFΔ).Western blot was performed once to confirm FASN-NTF linker region is a degron as shown in Fig.4a. c, Western blot of tissue specific CTF expression controlled by auxin. Western blot was performed once to show expression. d, FASN-CTF alters gene expression of ced-3(-) null mutants pathway enrichments –log10Q value. Enrichment analysis was performed using Enrichment Analysis tool from WormBase (https://wormbase.org/tools/enrichment/tea/tea.cgi). e, Heatmap of genes in enriched pathways with FDR < 0.1. n = 3 biological replicates. Log2 fold change of ced-3(-) expressing CTF compared to ced-3(-) with no CTF. f, PCA analysis for targeted metabolomics of ced-3(-) expressing CTF (green) compared to ced-3(-) with no CTF (purple). g, PCA analysis for targeted metabolomics of wild type animals expressing CTF (green) compared to wild type with no CTF (orange). h, N-acetylcysteine (NAC) level in wild type animals with and without CTF expression. n = 5 biological replicates. Mean ± SD. i, Enrichment analysis of targeted metabolomics of ced-3(-) expressing CTF compared to ced-3(-) with no CTF using MetaboAnalyst (https://www.metaboanalyst.ca/) with SMPDB. j, heatmap of wild type animals expressing CTF under ER stress showing Log2FC of the same metabolites shown in Fig. 4j. n = 5 biological replicates. k, OCR analysis of ced-3(-) mutant expressing CTF compared to ced-3(-) with no CTF expression. n = 3 biological replicates. Mean ± SD. p values were calculated using unpaired, two-tailed t-test (h,k) or one-tailed Fisher’s exact test (i).
