Extended Data Fig. 2: Elevated ROS stress response in ced-3(-) and ced-4(-) animals.
From: Proteolytic activation of fatty acid synthase signals pan-stress resolution
a, representative images of GCS-1p::GFP (glutamate-cysteine ligase) stress reporter induction with 16 mM paraquat (PQT) treatment to induce ROS stress in ced-3(-) and ced-4(-) animals at different time points. scale bar, 1 mm. b, digital zoom in of 0 h and 16 h of neuronal and intestinal GCS-1p::GFP reporter induction in ced-3(-) animals under ROS stress. scale bar, 200μm. c, quantification of GCS-1p::GFP stress reporter induction under ROS stress at early time points. n = 50 animals. d, quantification of GCS-1p::GFP stress reporter induction for both ced-3(-) and ced-4(-) animals. n = 40 animals. e-f, quantification of motile animals for ced-3(-) (e) and ced-4(-) (f) mutants with sodium chloride (NaCl) treatment to induce osmotic stress. ced-3(-) n = 3 and ced-4(-) n = 4 biological replicates. Mean ± SD. g-h, survival for ced-3(-) (g) and ced-4(-) (h) mutants with increasing concentration of PQT to induce ROS stress. n = 3 biological replicates. Mean ± SD, total animals examined in g, 0 mM, n = 532; 2 mM, n = 579, 4 mM, n = 493, 8 mM, n = 567; total animals examined in h, 0 mM, n = 505; 2 mM, n = 567, 4 mM, n = 286. *, p < 0.05; **, p < 0.0001. Exact p value see Source Data. For c,d, each circle represents one animal. Mean pixel intensity of each animal was normalized to mean value of wild type at 0 hr (no stress) and plotted as fold change. Violin plots show median (solid line) with quartiles (dashed line). p values were calculated using Mann-Whitney, two-tailed test (c,d) and unpaired two-tailed t-test (e,f,g,h).
