Extended Data Fig. 3: AgRP or POMC neurons do not mediate neuronal effects of inceptor on glucose homeostasis.
Inceptor immunoreactivity in the hypothalamus of 20 week old C57BL/6J Agrp-Cre+/− Iirwt/wt (WT) and Agrp-Cre+/− Iirflx/flx mice (KO). Scale bar is 100 µm (a). b–e, Body weight (b), as well as lean (c) and fat (d) tissue mass and fasting glucose (e) in 30 week old C57BL/6J WT and KO mice (n = 9 WT, n = 10 KO). f,g, Intraperitoneal glucose tolerance (f) and area under the curve (AUC) (g) in 30 week old male C57BL/6J WT and KO mice on a HFD (n = 8 WT, n = 9 KO). h,i, Intraperitoneal insulin tolerance (h) and AUC (i) in 31 week old male mice on a HFD (n = 8 WT, n = 9 KO). j, Inceptor immunoreactivity in the hypothalamus of 20 week old C57BL/6J Pomc-Cre+/− Iirwt/wt (WT) and Pomc-Cre+/− Iirflx/flx (KO) mice. Scale bar is 50 µm. k–n, Body weight (k), as well as lean (l) and fat (m) tissue mass and fasting glucose (n) in 30 week old male C57BL/6J WT and KO mice on a HFD (n = 9 each genotype). o,p, Intraperitoneal glucose tolerance (o) and area under the curve (AUC) (p) in 30 week old male C57BL/6J WT and KO mice on a HFD (n = 9 each genotype). q,r, Intraperitoneal insulin tolerance (q) and area under the curve (AUC) (r) in 31 week old C57BL/6J WT and KO mice (n = 9 each genotype). Asterisks indicate ** p < 0.01. Data present mean ± SEM. Data in panel C, E, G, I, L, M, N, P, R were analyzed by students two-sided, two-tailed test. Data in panel D were analyzed using the Mann-Whitney Test. Data in panel B, F, H, K, O, Q were analyzed by two-way ANOVA with Bonferroni post-hoc comparison for individual time points. P-value for group differences is p < 0.00377 (E). Corr., corrected.
