Extended Data Fig. 4: An inducible model of colonic EEC loss recapitulates the hyperphagia induced obesity phenotype.
From: Interaction between the gut microbiota and colonic enteroendocrine cells regulates host metabolism
(a) Schematic representation of the inducible colonic EEC deficiency model. Created with BioRender.com. (b) Representative genotyping gels of novel Neurog3 floxed allele. (c) Targeting strategy for generation of novel Neurog3 conditional floxed allele. (d) qRT-PCR of Neurog3 along regions of the mouse bowel (10 weeks of age, n = 3 mice). Statistics derived from biological replicates. (e) Body weight curve and (f) food intake for vehicle (Veh) or tamoxifen (TMX) treated iEECΔCol male mice on a regular diet (n = 8 mice). (g) Body weight curve and (h) food intake for iEECΔCol or tamoxifen (TMX) treated non-Cre control mice on a regular diet (n = 7 No Cre or 8 iEECΔCol mice). Mean values are represented by bars and individual values by jitter plots. Error bars are ± s.e.m. Two-tailed unpaired t-test used for all pairwise comparisons. *P < 0.05, **P < 0.01, ***P < 0.001.
