Extended Data Fig. 2: Acutely isolated Gq-NGOxtr neurons depolarize during CNO bath application.
From: Mechanosensation of the heart and gut elicits hypometabolism and vigilance in mice
(a) Gq-NGOxtr neurons expressing mCherry acutely dissociated from the mouse NG are visualized with DIC and epifluorescence microscopy. (b, non-glowing, top) Representative whole-cell current clamp recordings of control NG neurons or (red glowing, bottom) neurons expressing Gq-mCherry. After a 60-s baseline recording, CNO (10 µM) was bath applied for 5 min. (c, left) Average membrane potentials of neurons at baseline and CNO were significantly different between groups (2-Way ANOVA). Multiple comparisons demonstrate that control neurons exposed to CNO failed to depolarize (Tukey post hoc) while CNO significantly depolarized Gq-DGOxtr neurons (Tukey post hoc). (c, right) The same data as (c, left) expressed as the Δ membrane potential during CNO application. CNO significantly depolarized membrane potential in Gq-NGOxtr compared to control neurons (unpaired, two-tailed t-test). Center line is median, edges show quartiles, and error bars are min/max. Recordings acquired from a total of 6 Gq and 5 Control neurons; n = 2 mice.
