Extended Data Fig. 3: Loss of Kdm2a promotes lipid consumption in HFD induced mice.

(a) The quantitative analysis of Fig. 3f and (b) Fig. 3g. (c) Food and water intake monitoring of metabolic experiments (n = 8 per group). (d) Muscle mass analysis of Kdm2a ^fl/fl and Kdm2a^SKO male mice on 16-week HFD (n = 6 per group). (e) Representative H&E staining images in Gas tissue cross sections of HFD-fed Kdm2a ^fl/fl and Kdm2a^SKO male mice, average myotubes area analysis (right), Arrows indicated the blood vessels, Scale bars, 100 μm, (n = 6 per group). (f) Representative immunostaining of Laminin (green), CD31 (red) and DAPI (blue) in Gas tissue cross sections of HFD-fed Kdm2a ^fl/fl and Kdm2a^SKO male mice, CD31⁺ number analysis (right), Scale bars, 50 μm, (n = 5 per group). (g) RT-qPCR analysis of Mb, Vegfa and Myh7 expression in HFD-fed Kdm2a ^fl/fl and Kdm2a^SKO male mice (n = 6 per group). (h) Related fluorescence intensity analysis of Fig. 3p (n = 4 per group). (I) Representative immunostaining of Laminin (green), MHC II (red) and DAPI (blue) in Ta tissue cross sections of HFD-fed Kdm2a ^fl/fl and Kdm2a^SKO male mice. Scale bars, 100 μm, (n = 4 per group). (j) Treadmill endurance monitoring, electric shock times (left) (n = 6 per group) and exhaustion time (right) of HFD-fed Kdm2a ^fl/fl and Kdm2a^SKO male mice (n = 6 per group). (k) The mtDNA copy number analysis of HFD-fed Kdm2a ^fl/fl and Kdm2a^SKO male mice (n = 6 per group). (l-p) Kdm2a-WT and Kdm2a-KO C2C12 myotubes were stimulated with PA for 48h, and then (l) Flow cytometry analysis of the thermosensitive fluorescent dye stained ERthermAC-positive cells (n = 3 per group), western blot analysis of Pgc1, Myh4, Myh7(m), Sdhb, Ndufs1, Ndufs3(o) (n = 4 per group). Protein quantitative analysis (right). (n) RT-qPCR analysis of Mb, Vegfa and Myh7 expression (n = 6 per group). (p) Relative mtDNA copy number analysis (n = 6 per group). Data were expressed as mean ± SEM. Two-sided unpaired student’s t-test was used for statistical analysis (a-p).

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