Fig. 2: Enhanced glucose consumption in Kdm2a-KO mice under cold insult.
From: Kdm2a inhibition in skeletal muscle improves metabolic flexibility in obesity
a, Core body temperature monitoring of Kdm2afl/fl and Kdm2aSKO mice at 4 °C after cold adaptation (n = 6 per group). AUC, area under the curve. b, Random core body temperature of Kdm2afl/fl and Kdm2aSKO mice (n = 6 per group). c, Body weight before and after cold adaptation of Kdm2afl/fl and Kdm2aSKO mice (n = 6 per group). d, Fasting blood glucose of cold-adapted Kdm2afl/fl and Kdm2aSKO mice (n = 8 per group). e, Intraperitoneal glucose tolerance test and f, insulin tolerance test of cold-adapted Kdm2afl/fl and Kdm2aSKO mice (n = 6 per group). g–j, Parameters measured in metabolic cages during day and night conditions in Kdm2afl/fl and Kdm2aSKO mice: average energy expenditure (g), oxygen consumption (VO2) (h), Carbon dioxide emission (i) and RER (j) (n = 8 per group). k, Representative image of Gas tissue from Kdm2afl/fl and Kdm2aSKO mice (n = 6 per group). l, RT–qPCR analysis of Mb and vascular endothelial growth factor (Vegfa) expression in Gas tissues from Kdm2afl/fl and Kdm2aSKO mice (n = 6 per group). m, Western blot analysis of Myh4, Pgc1a and Myh7 in Gas tissues from cold-adapted Kdm2afl/fl and Kdm2aSKO mice (n = 4 per group). n, Representative immunofluorescence staining of Laminin (red), MHC I (green) and DAPI (blue) in frozen sections of Gas tissues from Kdm2afl/fl and Kdm2aSKO mice. Scale bars, 50 μm; n = 4 per group; intDen, integrated density. o, Endurance capacity test showing time to exhaustion (left), electric shock times (middle) and distance to exhaustion (right) of cold-adapted Kdm2afl/fl and Kdm2aSKO mice (n = 6 per group). p, Western blot showing expression of mitochondrial biogenesis-related genes in Kdm2afl/fl and Kdm2aSKO mice (n = 4 per group). q, Relative mitochondrial DNA (mtDNA) copy number analysis of cold-adapted Kdm2afl/fl and Kdm2aSKO mice (n = 6 per group). r, Representative TEM micrographs of Gas tissues from Kdm2afl/fl and Kdm2aSKO mice. Mitochondria are indicated by arrows. Scale bars, 10 μm, n = 4 per group. Data are expressed as mean ± s.e.m. Statistical significance was assessed by two-way ANOVA (a, e, h, i) followed by Bonferroni’s multiple comparisons test or two-sided Student’s t-test (b–d, g, j, l–q).
