Extended Data Fig. 2: Characterization of the Brca2^null cell line.

a-b Representative images of parental (a) and Brca2^null (b) CT26 cell karyotype analysis demonstrating increased double strand breaks. c, Quantification of karyotype analysis shown in a representing n=100 metaphases analyzed demonstrating the percent of metaphase nuclei containing at least 1 of indicated abnormality. Data represent results from a single experiment and were repeated twice with similar results. d, Quantification of karyotype analysis in 4T1 cells shown in Fig. 2d representing n=100 metaphases analyzed demonstrating the percent of metaphase nuclei containing at least 1 of indicated abnormality. Data represent results from a single experiment and were repeated twice with similar results. e, Quantification of immunofluorescent images of parental and Brca2^null CT26 murine colorectal adenocarcinoma cells 4 hours after 10 Gy irradiation stained with DAPI and antibodies to Rad51 and gamma-H2ax. f, In vitro relative viable cell count of parental and Brca2^null CT26 cell lines in the presence of PARP inhibitor olaparib at indicated concentrations after 96 hours in n = 3 independent assays in technical triplicate. Data are presented as mean values +/- SEM. Data represent results from a single experiment and were repeated three times with similar results. g-h, Tumor growth curve of parental and additional Brca2^null clones with ICB treatment in both the 4T1(g) and CT26 models (h). (* p<0.05, ** p<0.005,*** p<0.0005, **** p<0.00005). All p values represent two-sided unpaired t test at respective timepoint. Data are presented as mean values +/- SEM, n = 15 mice per group.