Extended Data Fig. 5: A computational procedure to identify candidate TRs controlling the gene expression program of GSCs.
From: Single-cell analyses reveal YAP/TAZ as regulators of stemness and cell plasticity in glioblastoma
a, Overview of the experimental flow for inference of the master Transcriptional Regulators (TRs) of the GSC state using the Rhabdomant pipeline on the Darmanis sc-RNA-seq dataset of primary GBM samples. See Methods for details. b, List of candidate master Transcriptional Regulators (TRs) emerging from the analysis of the Darmanis dataset of scRNA-seq dataset with the Rhabdomant pipeline, ordered on the base of their normalized enrichment signal (NES). The Rhabdomant pipeline calculated FDR adjusting for multiple comparisons; see Methods for details about p-value and FDR calculation. The lists of candidate master TRs of the GSC and of the DGC state are highlighted in orange and in light blue, respectively. The most significant candidate master TRs of the GSC state are indicated in red.
