Extended Data Fig. 6: In CDK4/6-R cells, CDK4/6 degraders fail to degrade CDK6 due to weak binding of compound. | Nature Cancer

Extended Data Fig. 6: In CDK4/6-R cells, CDK4/6 degraders fail to degrade CDK6 due to weak binding of compound.

From: Distinct CDK6 complexes determine tumor cell response to CDK4/6 inhibitors and degraders

Extended Data Fig. 6

a, Calu6 cells transiently expressing pcDNA3 (Ev) or pcDNA3-Flag-CRBN were treated with increasing concentrations of MS140 for 24 hr. Lysates were subjected to immunoblotting with the indicated antibodies. b, MV4-11 and A375 were treated MS140 (3 nM) or YKL-06-102 (3 nM) or BSJ-02-162 (3 nM) at different time points. Lysates were subjected to immunoblotting with the indicated antibodies. c, KMS-12-PE and Calu6 were treated MS140 (3 nM) or YKL-06-102 (3 nM) or BSJ-02-162 (3 nM) at different time points. Lysates were subjected to immunoblotting with the indicated antibodies.

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