Extended Data Fig. 2: Supportive Data to Main Fig. 2.
From: A tumor-derived type III collagen-rich ECM niche regulates tumor cell dormancy
(a) ECM enrichment pipeline for mass spectrometry. (b) ECM-enrichment validation by western blot before mass spectrometry analysis. Removal of intracellular components and ECM enrichment via sequential decellularization (lanes 2-4) from the total tissue lysate (1) was monitored by immunoblotting for actin (cytoskeleton protein) and histones (nuclear proteins). The remaining insoluble fraction (5) was highly enriched for ECM proteins (collagen I) and largely depleted for intracellular components. (c) Proportion of the mass-spectrometric signal intensity from matrisome (blue) and non-matrisome (grey) peptides for each sample, related to Supplementary Table 1b. (d) Masson’s trichrome staining of proliferative and dormant mice tumors. Scale bars, 50μm. (e) Percentage of tumor-derived and stroma-derived ECM d in D-HEp3 and T-HEp3 mice tumors, related to Supplementary Tables 1h, i. (f) Collagen III staining specificity tested in immunohistochemistry staining on human skin tissues (Scale bar, 100μm) and by western blot using purified native human collagen I and III.
