Extended Data Fig. 9: BACH1 suppresses tumor growth in p53 wild-type cell line HCT116-derived xenograft tumors.
HCT116 BACH1 inducible cells were made from HCT116 BACH1-/- cells by transfecting BACH1 inducible plasmid. HCT116 inducible cells were pre-incubated with or without doxycycline (Dox) for 48 h in vitro and subcutaneously injected into BALB/c nude mice and mice were fed with or without doxycycline diet. a, Western blot analysis of SLC7A11, BACH1 and p53 expression in HCT116 BACH1 inducible cells in the presence or absence of doxycycline. The experiment was repeated three times with similar results. b, Tumors isolated from nude mice implanted with HCT116 BACH1 inducible cells fed with or without doxycycline diet. Mice were sacrificed at day 20. n = 10 tumors. c, Tumor weights. n = 10 tumors. P value: <0.0001. d, Representative images of 4-HNE staining of HCT116 xenograft tumors. Bar scale=20μm. e, Quantification of 4-HNE staining using the immunoreactive score (IRS) system. n = 6 fields from 6 tumors. P value: <0.0001. P values were calculated by two-tailed Student’s t-test. ***p < 0.001; Data represent mean + S.E.M.
