Extended Data Fig. 8: eFT508 restores T cell activation in the Ptenpc−/−;Trp53pc−/− mouse model. | Nature Cancer

Extended Data Fig. 8: eFT508 restores T cell activation in the Ptenpc−/−;Trp53pc−/− mouse model.

From: The Akt/mTOR and MNK/eIF4E pathways rewire the prostate cancer translatome to secrete HGF, SPP1 and BGN and recruit suppressive myeloid cells

Extended Data Fig. 8

a, Representative FACS plots of the CD45+/CD3 population and CD45+/CD3+/CD8+ cells upon isotype control and anti-CD8 depleting antibody. b, Representative FACS plots of the CD45+/CD11b+ population and CD45+/CD11b+/Ly6Ghigh/Ly6Clow (PMN-MDSCs) upon isotype control and anti-Ly6G depleting antibody. c, Ifng, GrzmB and Foxp3 mRNA levels in eFT508-treated and vehicle-treated Pten−/−;Trp53−/− (RapidCap) allografts determined by qRT- PCR (vehicle group, n = 4; eFT508 group, n = 4, n = 5, n = 5 mice, respectively). Data are mean ± SD. Statistical analysis (unpaired two-sided Student’s t-test). d, Growth curve analysis of Pten−/−;Trp53−/− (RapidCap) murine prostate cancer cells, LnCap and PC3 human prostate cancer cell line treated with vehicle or 1 μM, 2 μM, 5 μM eFT508, determined by the Incucyte system. Data are mean ± SEM. The experiment was repeated two independent times with similar results.

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