Extended Data Fig. 1: The immunosurveillance of murine liver cancer.
From: Tumor-specific cholinergic CD4+ T lymphocytes guide immunosurveillance of hepatocellular carcinoma
a Schematic diagrams of the plasmids used to simultaneously induce CRISPR/Cas9-mediated deletion of Trp53 and Pten, Cre expression, and Myc overexpression in Rosa26Confetti/+ reporter mice. b Representative fluorescence microscopy images depicting (left) a monoclonal and (right) a polyclonal tumor from the livers of the mice in (a). Clonality was determined based on the expression of one or multiple fluorescent markers encoded by the Rosa26Confetti reporter, representative of one experiment. c Quantification of monoclonal and polyclonal tumors expressing the indicated fluorescent markers in livers from the mice in (a). Numbers of tumor nodules expressing the indicated fluorescent marker(s) are labeled in the pie plot. Data are from examination of 113 tumor nodules in liver sections from 10 Rosa26Confetti reporter mice. d Gating strategy used for flow cytometric analysis of murine hepatic immune cells. e Percentage of OX40+ CD4+ T cells in livers on the indicated days of liver cancer development. Each dot represents an individual mouse (n=6 mice per group). Data are the mean ± SEM. P values were determined by unpaired, two-tailed t-test. f Representative immunofluorescence images showing CD3 and CD11b expression in adjacent sections of an HCC-bearing liver, representative of one experiment. g Representative macroscopic images (upper) and microscopic images (lower) of tumor-bearing livers resected at the humane endpoint from NSG and control mice subjected to HCC induction, representative of one experiment. Related to Fig. 1.
