Extended Data Fig. 4: CCR7 mediates the interplay between LECs and GSCs.
a, Differential overall survival of glioblastoma patients stratified by different CCR6 expressions in the TCGA glioblastoma dataset. P values were calculated using log-rank test. b, Differential overall survival of glioblastoma patients stratified by CXCR4 expression in TCGA glioblastoma data. P values were calculated using log-rank test. c, mRNA levels of top 5 upregulated chemokine receptors in GSCs and NSCs analyzed by qPCR assay. Data are presented as mean ± SD from 3 independent experiments. P values were calculated using two-tailed unpaired Student’s t-test. d, Differential expression of CCR7 between normal and glioblastoma patients in Gravendeel data. GBM (n = 159) vs. normal brain (n = 8). P values were calculated using two-tailed unpaired Student’s t-test. e, Differential expression of CCR7 between normal and glioblastoma patients in TCGA glioblastoma dataset. GBM (n = 528) vs. normal brain (n = 10). P values were calculated using two-tailed unpaired Student’s t-test. f, CCR7+SOX2+/SOX2+ cells in both GSCs and NSCs were detected (n = 5). P values were calculated using two-tailed unpaired Student’s t-test. g, FACS dot plots showing the CD133− CCR7+ and CD133− CCR7− DGC2907. 96.3% of CD133− DGC2907 were CCR7 negative, 95% CI. h, FACS dot plots showing CD133-CCR7+ and CD133−CCR7− DGC20. 96.3% of CD133− DGC2907 were CCR7 negative, 95% CI. i, Spheroid formation assay was performed for GSCs transduced with either shCONT, shCCR7.291, or shCCR7.183 and co-cultured with LECs. n = 6 each group. j, Statistics for spheroid formation assay were performed for GSCs expressing shCONT, shCCR7.291, or shCCR7.183 co-cultured with LECs. Data are presented as mean ± SD from six independent experiments. P values were calculated using two-tailed unpaired Student’s t-test. k and l, Representative FACS dot plots showing CCR7high and CCR7low GSC2907(k) and GSC20(l). For box-and-whisker plot, the box indicates interquartile range (IQR), the line in the box indicates the median, the top of whiskers indicates the maxima and the bottom of the whiskers indicate the minima, Q1 and Q3 denote the first and third quartiles, respectively.
