Extended Data Fig. 7: Conditioned media from Hcmel12 cells does not stimulate STAT1 in BMDC and splenocyte cultures.
a Immunoblot of STAT1 and pSTAT1 isoforms from conditioned BMDCs. Representative image of three biological replicates is shown. b Densitometric ratio of normalised pSTAT1 relative to media in BMDC cultures (n = 3 biological replicates). c Heatmap of mean fluorescence intensity of specific activation markers of immune cells within BMDC cultures (n = 9 technical replicates over n = 3 biological replicates). Statistics are shown relative to negative control. d Immunoblot of STAT1 and pSTAT1 isoforms from conditioned splenocytes. Representative result of three biological replicates is shown. e Densitometric ratio of normalised pSTAT1 relative to media in splenocyte cultures (n = 3 biological replicates). f Heatmap of mean fluorescence intensity of specific activation markers of immune cells within splenocyte cultures (n = 9 technical replicates over n = 3 biological replicates). Statistics are shown relative to negative control. g Heatmap of metabolite abundance changes relative to wild-type tumors for respective tumor lineages (n = 6–38 individual tumours). Macrophages: Cd11b+ Ly6C- F4/80+. Monocytes: CD11b+ Ly6C+ F4/80-. Neutrophils: CD11b+ Ly6C+ Ly6G+. cDCs (conventional Dendritic Cells): CD11c+ MHCII+ F4/80- Ly6C-. All P-values were determined using a one-way ANOVA test with Fisher’s LSD Test (B-C,E-G). Error bars indicate SD. Measure of centrality is mean. * P = < 0.05, ** P = <0.01, *** P = <0.001, **** P = <0.0001. Heatmap representations of data where asterisks are not present report non-significant changes.
