Extended Data Fig. 3: ADAR2 did not functionally compensate for the effect of ADAR1 loss.

a, Protein level of ADAR2 in 22Rv1 cells that were infected with shNC or shADAR2 plasmid (single experiment). b, Volcano plot of significantly affected genes in 22Rv1 shADAR2 group relative to shNC group revealed by RNA-seq. Both shADAR1 and shNC groups contain two biological replicates, assessed as one experiment. P values were calculated using R v4.0.3 software. Statistical analysis was performed using two-tailed unpaired t-test. c, GSEA shows upregulated or downregulated pathways in shADAR2 cells compared to shNC. Statistical analysis was performed using two-sided permutation test, and P values were adjusted by Benjamini-Hochberg method. d, Histogram of number of various types of editing events in transcriptome-wide of 22Rv1 shADAR2 and shNC samples. n = 2 independent RNA-seq samples per group, assessed as one experiment. e, Expression level of ADAR1 and ADAR2 in DU-145 and 22Rv1 ADAR1 KD and control cells measured by RNA-seq. n = 2 independent RNA-seq samples per group, assessed as one experiment.