Extended Data Fig. 1: A single-cell landscape of primary tumors and peritoneal metastases in colorectal cancer.
a, Expression levels of signature genes in major cell clusters and subclusters. b, Comparison of the proportions of major cell clusters in normal peritoneal tissues, peritoneal metastases, normal colonic tissues, and CRC tissues from 12 patient. Boxes indicate medians and interquartile ranges (IQRs), and whiskers indicate the minimum and maximum values; two-tailed Wilcoxon test. c, Expression of representative marker genes in four subsets of macrophages within myeloid cells. d, Selected pathway activities in different subsets of macrophages. The pathway activities were assessed per cell using gene set variation analysis (GSVA).e, UMAP visualization of RNA velocity of seven subsets of macrophages and monocytes in peritoneal metastases. f, mIHC validation of macrophage subset Macrophage-FN1 present in peritoneal metastasis tumor tissues. Scale bars, 20 μm. Images are representative of n = 12 experiments. g, Spatial distribution(representative images of n = 12 experiments) of RGS5+ Pericyte cells, ACTA2+ fibroblast cells, CD66+ neutrophils, CD1C + cDC2 cells and TPASB1+ mast cells in CRC tissue and PM tissue. Scale bars, 20 μm. h, Quantitative proportions of RGS5+ Pericytes, TPASB1+ mast cells, ACTA2+ fibroblasts and CD1C + cDC2 cells in CRC tissue vs. PM tissue by quantification mIHC in 10 patients with PM and without liver metastases. For panels a-d and h: CRC, n = 12 patients; PM, n = 12 patients; normal colon, n = 12 patients; normal peritoneal, n = 9 patients. unpaired two-sides t-test. Data are presented as mean values +/- SD.
