Extended Data Fig. 4: Murine αVHH CAR T cells are well tolerated by immunocompetent mice. | Nature Cancer

Extended Data Fig. 4: Murine αVHH CAR T cells are well tolerated by immunocompetent mice.

From: Sensitizing solid tumors to CAR-mediated cytotoxicity by lipid nanoparticle delivery of synthetic antigens

Extended Data Fig. 4

(a) Blood serum analysis 7 d post i.v. administration of αVHH CAR T cells, untransduced T cells, or saline into naïve C57BL6/J mice. One-way ANOVA, mean ± s.d., n = 4 mice.; n.s. = not significant. (b) Body weight measurements following i.v. administration of αVHH CAR T cells, wild-type T cells, or saline into naïve C57BL6/J mouse. Two-way ANOVA, mean ± s.d., n = 5 mice per cohort; n.s. = not significant. (c) PBMC analysis before and 3 days after ACT of saline, WT, αVHH CAR, or αCD19 CAR T cells into C57BL6/J mice. One-way ANOVA; mean ± s.e.m., n = 4 mice for αCD19 CAR treated mice, n = 5 mice in all other cohorts; n.s. = not significant ***p = 0.0002. (d) Blood serum analysis 3 d post i.v. administration of saline, WT, αVHH CAR, or αhHER2 CAR T cells into naïve B6-HER2 mice. One-way ANOVA; mean ± s.e.m., n = 3 mice for hHER2 and VHH treatments, n = 4 mice for Saline and WT treatments.; *p = 0.01, **p = 0.0065.

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