Extended Data Fig. 6: Chidamide suppress SGs formation.
a, 3D representation of seven high-scoring candidate small molecules docking with RIOK1 identified using in silico analyses (related to Fig. 7a). b, Schematic representing the development of the HCC PDX models in NSG-nude mice and experimental treatment schemes using chidamide. c-d, Images (n = 2 images, 3 biological replicates) were analyzed to assess the average number of SGs/SG-positive cell based on G3BP1 immunofluorescence (c) and the fraction of PTEN mRNA in SGs (d) (related to Fig. 7l). e-g, Representative confocal images of staining against RIOK1 (green), G3BP1 (red) and PTEN mRNA (purple) in HCCLM3 cells cultured without GLN for 48 h in the absence and presence of 10 μM chidamide (e). Images (n = 2 images, 3 biological replicates) were analyzed to assess the average number of SGs/SG-positive cell based on G3BP1 immunofluorescence (f) and the fraction of PTEN smFISH in SGs (g). In c, d, f and g, error bars indicate mean ± SD for 3 independent experiments in which 2 fields of view. Data were analyzed by unpaired, two-tailed Student’s t-tests.
