Extended Data Fig. 6: Hypoxic cell cycle arrest in BV2 cells.
From: Hypoxia compromises the mitochondrial metabolism of Alzheimer’s disease microglia via HIF1
a, b, d, Cell cycle analysis using propidium iodide (PI) staining of BV2 cells exposed to normoxia (N: 21% O2, 48 h, a, left graph), hypoxia (H: 1% O2, 48 h, a, right graph), reoxygenation (R: 24 h H and 24 h N, b), or DMOG (D; 0.1 mM 24 h). The quantification of the percentage of cells found in the different cell cycle phases is shown in (b) and (d) (n = 3 biological independent cultures). c, BV2 cells were treated with a scrambled siRNA (siControl) or siRNAs against Hif1a (siHIF1a), Epas1 (siEpas1) and HIF1a-Epas1 (siHIF1a/Epas1). Interfered cells were exposed to N, H (24 h), or D (24 h) and the mRNA levels of Hif1a (left graph) and Epas1 (right graph), and the cell cycle (lower row) were estimated by qRT-PCR (n is indicated between brackets, biological independent cultures). Data are represented as mean ± S.E.M.
