Extended Data Fig. 3: Hippocampal levels of Aβ plaques and Triton X-100 Aβ1-42 are not affected by anti-PD-L1 treatment in Trem2-/-5xFAD mice. | Nature Aging

Extended Data Fig. 3: Hippocampal levels of Aβ plaques and Triton X-100 Aβ1-42 are not affected by anti-PD-L1 treatment in Trem2-/-5xFAD mice.

From: Alzheimer’s disease modification mediated by bone marrow-derived macrophages via a TREM2-independent pathway in mouse model of amyloidosis

Extended Data Fig. 3

(a) Graphic presentation of our RAWM procedure. (b) Graphic presentation of our NOR procedure. (c) RAWM performance of Trem2+/+WT (n=9) and Trem2−/−WT mice (n=12) presented (Y-axis; mean ± SEM) as the number of errors made by the mice (upper panel) and latency to platform (sec, lower panel), in 3-trial bins (x axis) analyzed by two-way ANOVA with repeated measures yielding only main effect of trial bins (upper panel: F(9,171)=45.03, p < 0.0001, 95% CI -1.031-0.8198; lower panel: F(9,171)=66.04, p < 0.0001, 95% CI -6.329-5.767). (d, upper panel) ELISA assessment of Aβ1-40 TBS-soluble hippocampal fractions from anti-PD-L1 (n=9; 7 females (F), 2 males (M)), and IgG2b (n=9; 6 F, 3 M) treated Trem2−/−5xFAD and untreated Trem2−/−WT (n=7; 5 F, 2 M); data derived from 3 cohorts of mice, pooled together after normalization per cohort. (d, lower panel) ELISA assessment of Aβ1-40 Triton X-100-soluble hippocampal fractions from anti-PD-L1 (n=11; 9 F, 2 M) and IgG2b (n=11, 8 F, 3 M) treated Trem2−/−5xFAD and untreated Trem2−/−WT (n=9; 7 F, 2 M); data derived from 4 cohorts of mice, pooled together after normalization per cohort. (e) ELISA assessment of Aβ1-40 TBS-soluble (upper panel) and Triton X-100-soluble (lower panel) hippocampal fractions from anti-PD-L1 (n=7 F) and IgG2b (n=8 F) treated Trem2+/+5xFAD and untreated Trem2+/+WT (n=5 F); data derived from 3 cohorts of mice, pooled together after normalization per cohort. Data were analyzed using one-way ANOVAs followed by a Fisher’s LSD test: (d) TBS-soluble: F(2,22)=25.0, p < 0.0001, R2=0.6944; Triton X-100-soluble: F(2,28)=9.568, p < 0.0001, R2=0.6862; (e) TBS-soluble: F(2,17)=3.479, p=0.0008, R2=0.5653; Triton X-100-soluble: F(2,17)=3.214, p=0.0003, R2=0.623. No significant difference (n.s.) was found following Fisher’s LSD tests. Box plots represent the minimum and maximum values (whiskers), first and third quartile (box boundaries), median (box internal line), and mean (cross); data in all other graphs are shown as mean ± SEM.

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