Extended Data Fig. 4: Microglia from OxPC injected spinal cords express gene signatures comparable to other disease and aging associated microglia.
From: Single-cell and spatial RNA sequencing identify perturbators of microglial functions with aging
a-b) Dot plot comparing the expression of signatures genes from disease associated microglia (daMG2 in red, daMG3 in green, daMG4 in blue) that associate with experimental autoimmune encephalomyelitis lesions across the 8 MPH clusters identified by scRNAseq (a) or across the 4 different treatment conditions (b). c-d) Dot plot comparing the expression of white matter associated microglia (WAM) signature genes across the 8 MPH clusters (c) or across the 4 different treatment conditions (d). Dashed green lines highlight the MPH clusters that were most like WAMs. d-e) Dot plot comparing the expression of Alzheimer’s disease associated microglia (DAM) signature genes across the 8 MPH clusters (e) or across the 4 different treatment conditions (f). Dashed green lines highlight the MPH clusters that were most like DAMs. Dot plot comparing the expression of 540-day old microglia (OA2 in red, OA3 in blue) signature genes from the spinal cord across the 8 MPH clusters (g) or across the 4 different treatment conditions (h). The size of the dot corresponds to the percentage of cells expressing the gene in each cluster. The colour represents the average gene expression level. Sample size n = 3 per experimental group (each n represents a pool of 4 spinal cords for a total of 12 mice per group) and data are represented as mean ± SD.
