Extended Data Fig. 1: Microglia/macrophage functions are altered in the ageing exacerbated OxPC induced spinal cord lesions at day 3.
From: Single-cell and spatial RNA sequencing identify perturbators of microglial functions with aging
a-c) Graphs comparing spinal cord lesion spread (a), total lesion volume (b), and lesion epicenter area (c) between 6wk and 52wk mice 3 days after PAzePC injection, analyzed from EC and NR-stained tissue sections. d, g, j, m, q) Representative confocal images of the spinal cord lesions of 6wk and 52wk mice 3 days after PAzePC injection. Sections were labeled with DAPI (blue), E06 (green), IBA1 (red) (d); or with MBP (grey), NFH (red) (g); or with DAPI (blue), Tuj1 (green), cleaved caspase-3 (red) (j); or with CD16/32 (grey), IL-1β (green), Arg1 (red) (m); or with iNOS (green), IBA1 (red) (q). e-f, h-i, k-l, n-p, r-t) Graphs comparing the E06+ percent (e), IBA1+ percent (f), the number of NFH+ (h) or Tuj1+ axons (i), the number of cleaved caspase 3+ particles (k), CD16/32+ percent (l), IL-1β+ percent (n), CD16/32+ IL-1β+ over CD16/32+ proportions (o), iNOS+ percent (p), IBA1+ iNOS+ over IBA1+ proportions (r), Arg1+ percent (s), and CD16/32+ Arg1+ over CD16/32+ proportions (t) between 6wk and 52wk spinal cords within the lesion ROI at day 3. Data were acquired from 2 separate experiments, n = 6 per experimental group. Significance indicated as * p< 0.05, ** p< 0.01, two-tailed, unpaired t-test, comparing 6wk and 52wk mice. Data are represented as mean ± SD.
