Extended Data Fig. 6: H3K36 methylation is needed for establishing oncogene-induced senescence.
a, Expression levels of NSD2 (n = 4). b, Expression levels of NSD3 (n = 3). c, Expression levels of SMYD2 (n = 4). d, Single-cell nuclear intensity values for H3K36me3 staining 7 days after treatment with 4OHT or vehicle (DMSO) of IMR90 ER:RAS cells infected with different pGIPZ shRNAs against NSD2, NSD3, SMYD2, AHCY or the parental pGIPZ vector (n = 1,000 cells per condition for a representative experiment out of 3). The threshold used to quantify the cells stained for H3K36me3 cells in Fig. 5b, is shown as a red dashed line. All statistical significances were calculated using one-way ANOVA. All error bars represent mean ± s.d.
