Extended Data Fig. 10: 3DA improves the engraftment of umbilical cord blood cells.
a-d, GSEA signatures for the cord blood RNA-seq experiment. e, Cord blood-derived human hematopoietic stem and progenitor (CD34 + ) cells were treated at day 1, 4 and 7 with 10 μM 3DA or DMSO and analyzed before xenotransplantation at day nine. Absolute cell numbers were determined by manual cell counting. Data are represented as mean ± SD, n = 4 independent experiments. Statistical significance was calculated using one-tailed Student’s t test. f, 1.5 × 106 Cells from the experiment described in a were transplanted into NSG mice. The engraftment of human (CD45+) cells and the percentage of primitive cells (CD34 + CD38−) in the bone marrow was analyzed by flow cytometry. For e and f, each shape (open cicle, closed circle, star, square or triangle) represents a different cord blood sample. Each shape is the average of 2‐3 transplanted mice with that cord blood sample (n = 4 independent cord blood samples). Statistical significance was calculated using one-tailed Student’s t test. g, Gating strategy for the experiment shown in Fig. 7f. h, Gating strategy for the experiment shown in Fig. 7g.
