Extended Data Fig. 7: Expression of tra^F is necessary but not sufficient for the larger enterocyte size in female intestines.

a, Expression of tra^F in ECs in males did not affect cell size, neither did treatment with rapamycin (200 µM), at 20 days of age (control male mexG4 > + vs feminised male mexG4 > tra^F; scale bar = 10 µm; n = 6-8 intestines, n = 20-25 enterocytes per intestine, circles indicate individual values and diamonds represent the average value per intestine; linear mixed model, interaction p > 0.05; post-hoc test). b, Knock-down of tra^F in ECs in females reduced the size of enterocytes to the level of rapamycin-treated females, which was not further reduced by rapamycin treatment (200 µM), at 20 days of age (control female mexG4 > + vs masculinized female mexG4 > tra^{F [RNAi]}; scale bar = 10 µm; n = 6-8 intestines, n = 17-23 enterocytes per intestine, circles indicate individual values and diamonds represent the average value per intestine; linear mixed model, interaction p < 0.01; post-hoc test). For box-and-whiskers plots, Median, 25th and 75th percentiles, and Tukey whiskers are indicated.

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