Fig. 4: Bchs is a required target for autophagy activation, lifespan extension and intestinal homeostasis from the mTORC1–histone axis.

a, Knockdown of Bchs in ECs of adult females had no effect on lifespan, but it abolished the increase in lifespan in response to rapamycin (long-rank test, control versus RU486 P = 0.40, rapamycin versus rapamycin+RU486 P = 0.0065, n = 198–199 flies per condition). b, Knockdown of Bchs in ECs of adult males shortened lifespan (long-rank test, control versus RU486 P = 0.0095, n = 198-200 flies per condition). c, Knockdown of Bchs in ECs of adult females had no effect on lifespan but abolished the increase in lifespan in response to spermidine (long-rank test, control versus RU486 P = 0.54, spermidine versus spermidine + RU486 P = 0.012, n = 198–199 flies per condition). d, Knockdown of Bchs in enterocytes of adult males shortened lifespan (long-rank test, control versus RU486 P = 0.023, n = 199 flies per condition). See also Supplementary Tables 5 and 6.