Extended Data Fig. 7: Altre depletion in Treg cells leads to CD4⁺ and CD8⁺ T cell activation in the aged livers.

a, Representative flow cytometry plots of CD44 and CD62L staining in the liver CD4⁺ T cells isolated from Altre-WT and Altre-KO mice (14 months old). b, Statistical analysis of the percentage of CD62L⁺CD44⁻, CD62L⁺CD44⁺, and CD62L⁻CD44⁺ subsets in CD4⁺ T cell in the liver from Altre-WT and Altre-KO aged mice (n = 6) as in a. Two-tailed t-test. c, Representative flow cytometry plots of CD44 and CD62L staining in the liver CD8⁺ T cells isolated from Altre-WT and Altre-KO mice (14 months old). d, Statistical analysis of the percentage of CD62L⁺CD44⁻, CD62L⁺CD44⁺, and CD62L⁻CD44⁺ subsets in CD8⁺ T cell in the liver from Altre-WT and Altre-KO aged mice (n = 6) as in c. Two-tailed t-test. e, Statistical analysis of the percentage of CD45⁺CD4⁺ T cells in the spleen, liver, and VAT from Altre-WT and Altre-KO mice (14 months old; n = 6). Two-tailed t-test. f, Statistical analysis of the percentage of CD45⁺CD8⁺ T cells in the spleen (n = 6), liver (n = 6), and VAT (n = 5) from Altre-WT and Altre-KO aged mice (14 months old). Two-tailed t-test. g, Statistical analysis of the percentage of CD45⁺ γδ T cells in the spleen (Altre-WT, n = 6; Altre-KO, n = 6), liver (Altre-WT, n = 5; Altre-KO, n = 6), and VAT (Altre-WT, n = 4; Altre-KO, n = 4) from aged mice (14 months old). Two-tailed t-test. Data are mean ± s.d. NS, non-significant.

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