Extended Data Fig. 3: S100a8 or S100a9 knock-down down-regulates the LPS-induced immune response.
From: Transcriptional and epigenetic decoding of the microglial aging process
(a) Scheme of Lipofectamine transfection and LPS challenge to primary microglia. (b) qPCR reveals that siS100a8 transfection by Lipofectamine transfection successfully knocks down S100a8. Consequently, 7 out of 13 immune-related genes were down-reregulated upon LPS challenge. In the negative control group: S100a8 (N = 5), Ccl3 (N = 3), Ccl4 (N = 3), Ccl5 (N = 3), Ccl12 (N = 3), Cd14 (N = 3), Cxcl13 (N = 3), Ifitm2 (N = 5), Ifitm3 (N = 3), Il1b (N = 3), Il6 (N = 5), Myd88 (N = 3), Socs3 (N = 4) and Tnf (N = 3). In the siS100a8 group: S100a8 (N = 6), Ccl3 (N = 6), Ccl4 (N = 6), Ccl5 (N = 6), Ccl12 (N = 6), Cd14 (N = 6), Cxcl13 (N = 6), Ifitm2 (N = 9), Ifitm3 (N = 6), Il1b (N = 6), Il6 (N = 9), Myd88 (N = 6), Socs3 (N = 9) and Tnf (N = 6). Where “N” corresponds to the number of biological replicates. (c) qPCR reveals that siS100a9 transfection by Lipofectamine transfection successfully knocks down S100a9. Consequently, 8 out of 13 immune-related genes were down-reregulated upon LPS challenge. In the negative control group: S100a9 (N = 6), Ccl3 (N = 6), Ccl4 (N = 6), Ccl5 (N = 6), Ccl12 (N = 6), Cd14 (N = 5), Cxcl13 (N = 6), Ifitm2 (N = 6), Ifitm3 (N = 6), Il1b (N = 6), Il6 (N = 6), Myd88 (N = 6), Socs3 (N = 6) and Tnf (N = 6). In the siS100a9 group: S100a9 (N = 6), Ccl3 (N = 6), Ccl4 (N = 6), Ccl5 (N = 6), Ccl12 (N = 6), Cd14 (N = 6), Cxcl13 (N = 6), Ifitm2 (N = 6), Ifitm3 (N = 6), Il1b (N = 6), Il6 (N = 6), Myd88 (N = 5), Socs3 (N = 6) and Tnf (N = 6). Where “N” corresponds to the number of biological replicates. Data are presented as mean ± SD. Two-tailed independent t test.
