Fig. 7: 3xDR leads to cognitive decline and myelination impairment in non-aged mice.
From: Transcriptional and epigenetic decoding of the microglial aging process
a, Scheme of the 3xDR mice preparation and behavior tests. b, NOR reveals that 3xDR induces cognitive decline in recognition memory, resembling the phenotype of aged mice. Left, the paradigm of NOR; right, the mouse exploration time to two objects. n = 12, 10, 11 and 11 mice for young, aged, control and 3xDR groups, respectively. c, Y maze reveals that 3xDR induces cognitive decline in spatial learning. Left, representative trajectory heat map in the Y maze; middle and right, the statistical results of control and 3xDR mice. n = 10 mice for each group. d, Morris water maze reveals that 3xDR induces cognitive decline in spatial learning. Left, representative swimming route of control and 3xDR mice in the Morris water maze; right, latency in the training phase, the number of times the mice passed across the platform and the time in the target quadrant in the probe trial. n = 10 and 11 mice for control and 3xDR, respectively. e, Representative confocal images of MBP in the cortex and quantification of average MBP expression in control and 3xDR mouse brains. 3xDR impairs myelination in the cortex. n = 9 and 8 mice for control and 3xDR, respectively. Data are presented as mean ± s.d. Two-tailed independent t-test. Ctrl, control; OFT, open field test.
