Extended Data Fig. 9: Transcriptional Variability.

a–c) Transcriptional variability upon ageing (a), in pericentral and periportal zones (b) and in the differently ploid hepatocytes (c) expressed as Pearson Coefficient of all detected genes. d) Violin plots for differential dispersed and expressed genes in the Tabula Muris senis dataset. Dispersion and expression was calculated using BASiCS. Significance for the figures a-d was calculated using Wilcoxon test within geom_signif function. The lower and upper hinges of the boxplot correspond to the first and third quartiles (25th and 75th percentiles) while the middle line is median and the whiskers extend to 1.5 × interquartile range (IQR) from both lower and upper hinges. The notches extend 1.58 × IQR/sqrt(n), which is roughly 95% confidence intervals (CIs) for comparing medians e) Volcano plot for differentially dispersed genes as found in Tabula Muris senis data of flow sorted liver cells. f) Biological processes (upper panel) and Cellular components (lower panel) for differentially expressed and dispersed genes.