Extended Data Fig. 1: Lipid Remodeling in the ageing mouse liver.

a) PCA projection of bulk RNA-seq data¹¹ derived from young and old mouse livers. b) Differentially enriched Gene Ontology Biological Processes in the aged liver tissue derived from A (Supplementary Table 1). The colour scale represents the number of genes in each term and Adjusted p-value (Benjamini Hochberg) on x-axis is derived with over representation test using enrichGO function of clusterProfiler R package. c) Representative images from H&E (upper panel) and Sirius Red (lower panel) stainings on liver sections from a young and an old mouse. Scale bar = 100μm. d) Representative images of PP (periportal) and CV (central vein areas) of Oil-red-O (O-R-O, upper panel) and Plin2 immunostainings (lower panel) on liver sections from young and old mice. Scale bar = 100 µm. e) Spatial transcriptomics slides of the second biological replicate. f) PCA plot of the spatial data after integration of the four datasets using canonical correlation analysis. Different colours represent the different samples. g) PC plot showing the top 50 genes that separate the ageing groups in Supplementary Fig. 1e.