Extended Data Fig. 3: Lipids and sorting of hepatocytes based on zones.

a) PCA of lipidomic data coloured by age. b) Gating strategy for isolation of pericentral and periportal hepatocytes. c) qRT-PCR to validate the enrichment for pericentral and periportal hepatocytes based on expression ratios of Glul and Cyp2f2 levels. Shown are individual replicates for young and old mice (as indicated). d) Mitochondrial content of livers was measured using primers against genomic copies of mt-Cytb and b-actin. Individual values are given as dots. Error bars represent SEM. Statistical significance was determined using a two-tailed unpaired t-test.