Extended Data Fig. 1: Aging leads to expansion of memory CD8+ T cells in circulation.
(a) Schematic of experimental procedure to isolate blood and perform flow cytometry at 3 timepoints during atherogenesis in young wild-type, aged wild-type, and young Ldlr-/- mice. (b) Blood-cell flow cytometry gating strategy to identify CD8+ T cells. Live CD45+ lymphocytes were gated on CD3e+ cells, CD8+ cells, and subdivided into naive (CD62L+CD44−), central memory (CD62L+CD44+), and effector memory (CD44+CD62L−) then PD1+ and either granzyme K+ or Tox+ cells. (c-l) Flow cytometry quantification of T cell populations as a frequency of CD3+ T cells for young C57BL/6, aged C57BL/6, and young Ldlr-/-, mice at baseline, midway through the western diet feeding period (5-weeks), and just prior to sacrifice (10-weeks). N = 6 biological replicates per group over 1 independent experiment. Measurements were taken from distinct samples. Data in this figure represents mean ± SEM. 2-Way ANOVA with Tukey’s post-hoc test. PCSK9 = proprotein convertase subtilisin/kexin type 9 serine protease, AAV = adeno-associated virus, CM = central memory, EM = effector memory.
