Fig. 8: Pharmacological inhibition of CD38 improved female fertility in middle-aged mice.
a, Ovarian NAD+ levels in middle-aged mice with or without 78c treatment for 8 d (n = 16 mice for each group). b, Serum AMH levels in control and 78c-treated middle-aged mice for 3 weeks (n = 19–22 mice for each group). c, Mean litter size of middle-aged mice with or without 78c treatment (n = 13 or 14 mice for each group). d, Representative images of MII oocytes from superovulated control and 78c-treated middle-aged mice (n = 12 mice for each group). Arrows indicate oocytes with fragmentation. Scale bar, 100 μm. e, Mean ovulated oocyte numbers collected from control and 78c-treated middle-aged mice. f, The proportion of abnormal oocytes in each group (n = 8 mice for each group). g, Representative images of the spindle morphology and chromosome alignment in MII oocytes from control and 78c-treated middle-aged mice. Scale bar, 5 μm. h, The proportion of abnormal spindles in oocytes from each group (n = 9 mice for each group). Data are presented as the mean ± s.e.m. P value was determined by unpaired two-tailed t-test between the two groups.
