Fig. 2: Cell type-specific spatiotemporal changes in transcriptional regulatory programs throughout ovarian aging.
From: Spatiotemporal transcriptomic changes of human ovarian aging and the regulatory role of FOXP1
a, Representative KEGG pathways of upregulated DEGs (top) and downregulated DEGs (bottom) compared between the O/Y, M/Y and O/M groups in eight cell types. P values were calculated by Fisher’s exact test. b, Gene set score analysis of cellular senescence pathways in various ovarian cell types of different groups, Y, M and O. Data were analyzed by two-sided Wilcoxon rank-sum tests. Box-and-whisker plots show the minimum, 25th percentile, median, 75th percentile and maximum. n = 3 per age group. c, ST spot illustrating cellular senescence and SASP score within the human ovary. The red arrow indicates follicles. d, Lipofuscin staining of different aged human ovaries. IOD, integrated optical density. Data are presented as the mean ± s.e.m. n = 10 for each group (one-way ANOVA). e, Violin plot showing the expression of CDKN1A in all ovarian cell types. Data were analyzed by two-sided Wilcoxon rank-sum tests. f, ST spot indicating the CDKN1A expression in human ovaries of different ages. g, Multiplex IHC staining for CDKN1A, ZP3 (oocyte marker), GSTA1 (GC marker) and DCN (T&S cell marker) of differently aged human ovaries. The experiment was repeated three times. h, Cytokine oligonucleotide array for SASPs in human ovaries of different ages. n = 3 for each group.
