Extended Data Fig. 4: HFD-LOH mice modeling.
From: Targeting dysregulated phago-/auto-lysosomes in Sertoli cells to ameliorate late-onset hypogonadism
a, FACS profile showing unacidified lysosomes accumulated in the SC treated with Baf A for 12 h by LysoSensor probe. b, Body weight of mice with normal or high fat diet for 10 months. c, Ratio of testis weight to Body weight in ND or HFD-LOH mice (nND, HFD-LOH = 12 mice). d, Representative staining and quantification of VASA+ cell indicating atrophy of seminiferous tubules (nND, HFD-LOH = 20 ROI). e, Representative images of PPT1 level in SCs of ND or HFD-LOH mice(nND = 376 cells, nHFD-LOH = 345). f, Diagram and records of Rotarod assay in ND or HFD-LOH mice (nND, HFD-LOH = 8 mice). g, h, Schematic diagram showing the sexual behavior (g) including sniffing and mating (h) in ND or HFD-LOH mice (nND, HFD-LOH = 6 mice). At least five individual mice for each group. The boxes represent the median and interquartile range and the bars represent roughly 95% confidence interval for comparing medians (e). Data are presented as mean ± s.e.m (c, d, f, h). Comparison by two-tailed unpaired t-test. Scale bars: 50 μm (d, e).
